cell lines hek293t cells atcc crl3216 h vegf reporter 293 cell line genomeditech gm c09057 experimental models  (ATCC)

Journal: Clinical Science (London, England : 1979)

Article Title: The loss of glycoprotein nonmetastatic melanoma protein B (GPNMB) alters endothelial cell permeability, metabolism, and survival during infectious challenge

doi: 10.1042/CS20256682

Figure Lengend Snippet: (A ) Interleukin-8 (IL-8), ( B ) tumor necrosis factor-alpha (TNF-α), ( C ) the tissue inhibitors of metalloproteinases 1 (TIMP1) concentrations, and ( D ) metalloproteinases-2, ( E ) metalloproteinases-3, and ( F ) metalloproteinases-9 activity in the culture medium of human microvascular endothelial cells (HMVECs) treated with scrambled siRNA (SCR) or GPNMB-targeting siRNA (siGPNMB), following stimulation with either vehicle or heat-killed E. coli (HKEC, 3 × 10⁸ cells/ml) for 6 h. Each dot represents an individual sample. data are presented as mean ± SEM. Statistical analysis: Two-way ANOVA, followed by the Tukey post hoc test.

Article Snippet: The cells were cultured in Vascular Cell Basal Medium (ATCC; PCS-100–030), supplemented with Microvascular Endothelial Cell Growth Kit-VEGF (ATCC; PCS-110–041), penicillin (100 U/ml), and streptomycin (100 U/ml), at 37°C in a humidified 5% CO2 atmosphere.

Techniques: Activity Assay

Journal: Clinical Science (London, England : 1979)

Article Title: The loss of glycoprotein nonmetastatic melanoma protein B (GPNMB) alters endothelial cell permeability, metabolism, and survival during infectious challenge

doi: 10.1042/CS20256682

Figure Lengend Snippet: (A ) Transendothelial electrical resistance (TEER) of human microvascular endothelial cells (HMVECs) treated with scrambled siRNA (siControl) or GPNMB-targeting siRNA (siGPNMB). ( B ) TEER measurements following lipopolysaccharide (LPS, 100 ng/ml) exposure, normalized to baseline values prior to stimulation. ( C ) TEER measurements after exposure to heat-killed E. coli (HKEC, 3 × 10⁸ cells/ml), normalized to baseline values. ( D-F ) Protein expression levels of ICAM, Integrin-β, and VE-Cadherin, with α-Tubulin used as a loading control. The number of samples used in each experiment ( n ) is expressed in parentheses or dots. Data are presented as mean ± SEM. Statistical analysis: Two-way ANOVA, followed by the Tukey post hoc test.

Article Snippet: The cells were cultured in Vascular Cell Basal Medium (ATCC; PCS-100–030), supplemented with Microvascular Endothelial Cell Growth Kit-VEGF (ATCC; PCS-110–041), penicillin (100 U/ml), and streptomycin (100 U/ml), at 37°C in a humidified 5% CO2 atmosphere.

Techniques: Expressing, Control